Genotyping and primer details


Genotyping and primer details for 133F03 - At4g20170
Line ID 133F03
Locus At4g20170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10898515 of line 133F03 Chr4:10898515
   
Insertion allele amplicon 8474 - CD56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD56: 5'-TTACATGTTCCAGTGAAAGAGACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10897845
   
wt amplicon (over InsPos) 04ft - CD56
wt amplicon start Chr4:10897845
wt amplicon end Chr4:10898668
wt amplicon size 824 bp
Locus specific primer CD56: 5'-TTACATGTTCCAGTGAAAGAGACG-3'
2nd locus specific primer 04ft: 5'-GTTAAAAAGTTGACGACAAGGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11