Genotyping and primer details


Genotyping and primer details for 134B08 - At3g46420
Line ID 134B08
Locus At3g46420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17082899 of line 134B08 Chr3:17082899
   
Insertion allele amplicon 8474 - NR72
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NR72: 5'-CCGCGTTTCTTTAAGATGACTTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17083242
   
Insertion allele amplicon 3144 - NR73
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer NR73: 5'-CCTTTTTGTGTGCATGCTAGATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17082538
   
wt amplicon (over InsPos) NR72 - NR73
wt amplicon start Chr3:17082538
wt amplicon end Chr3:17083242
wt amplicon size 705 bp
Locus specific primer NR73: 5'-CCTTTTTGTGTGCATGCTAGATTA-3'
2nd locus specific primer NR72: 5'-CCGCGTTTCTTTAAGATGACTTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11