Genotyping and primer details


Genotyping and primer details for 134C06 - At5g65810
Line ID 134C06
Locus At5g65810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26338178 of line 134C06 Chr5:26338178
   
Insertion allele amplicon 8474 - 19fb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19fb: 5'-TGACAAGCTGGTTTGTATGAACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26337861
   
wt amplicon (over InsPos) 02gl - 19fb
wt amplicon start Chr5:26337861
wt amplicon end Chr5:26338509
wt amplicon size 649 bp
Locus specific primer 19fb: 5'-TGACAAGCTGGTTTGTATGAACTC-3'
2nd locus specific primer 02gl: 5'-TTGAATGAAGAAGAGACAGAAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11