Genotyping and primer details


Genotyping and primer details for 134C07 - At5g61200
Line ID 134C07
Locus At5g61200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24621288 of line 134C07 Chr5:24621288
   
Insertion allele amplicon 8474 - MF20
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MF20: 5'-TCGTTGTCAGAGTCTCGTTTAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24620836
   
wt amplicon (over InsPos) 05ua - MF20
wt amplicon start Chr5:24620836
wt amplicon end Chr5:24621553
wt amplicon size 718 bp
Locus specific primer MF20: 5'-TCGTTGTCAGAGTCTCGTTTAGAA-3'
2nd locus specific primer 05ua: 5'-ATGGAAACAATCTGGATTTACAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11