Genotyping and primer details


Genotyping and primer details for 134D01 - At1g22220
Line ID 134D01
Locus At1g22220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7846996 of line 134D01 Chr1:7846996
   
Insertion allele amplicon 8474 - BG44
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BG44: 5'-ATTGGTTTTTGGCTTATTCTTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7846524
   
wt amplicon (over InsPos) 20sq - BG44
wt amplicon start Chr1:7846524
wt amplicon end Chr1:7847623
wt amplicon size 1100 bp
Locus specific primer BG44: 5'-ATTGGTTTTTGGCTTATTCTTCCT-3'
2nd locus specific primer 20sq: 5'-CTCTAAAGCGTTCTTCTTGCACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11