Genotyping and primer details


Genotyping and primer details for 134E12 - At5g18290
Line ID 134E12
Locus At5g18290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6056309 of line 134E12 Chr5:6056309
   
Insertion allele amplicon 8474 - ML21
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer ML21: 5'-GAAACAAATATGCCAGTGGGTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6055764
   
wt amplicon (over InsPos) 52gc - ML21
wt amplicon start Chr5:6055764
wt amplicon end Chr5:6056817
wt amplicon size 1054 bp
Locus specific primer ML21: 5'-GAAACAAATATGCCAGTGGGTACT-3'
2nd locus specific primer 52gc: 5'-ATAGTCAGAGCTCACCAAAAAGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11