Genotyping and primer details


Genotyping and primer details for 136F09 - At5g43330
Line ID 136F09
Locus At5g43330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17391506 of line 136F09 Chr5:17391506
   
Insertion allele amplicon 8474 - GG31
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GG31: 5'-AGTGTGTTTCTGTTATTGTGCAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17390895
   
wt amplicon (over InsPos) 06dk - GG31
wt amplicon start Chr5:17390895
wt amplicon end Chr5:17391813
wt amplicon size 919 bp
Locus specific primer GG31: 5'-AGTGTGTTTCTGTTATTGTGCAGG-3'
2nd locus specific primer 06dk: 5'-TTGCTAACATACATGAGCATCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11