Genotyping and primer details


Genotyping and primer details for 136H02 - At5g47430
Line ID 136H02
Locus At5g47430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19236666 of line 136H02 Chr5:19236666
   
Insertion allele amplicon 8474 - 04jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04jr: 5'-CCTTTCTTCGTTTACCAGAAGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19236076
   
wt amplicon (over InsPos) 18jr - 04jr
wt amplicon start Chr5:19236076
wt amplicon end Chr5:19236957
wt amplicon size 881 bp
Locus specific primer 04jr: 5'-CCTTTCTTCGTTTACCAGAAGATG-3'
2nd locus specific primer 18jr: 5'-CTCCTTTTGTTTGACTCATCTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11