Genotyping and primer details


Genotyping and primer details for 138D02 - At5g64070
Line ID 138D02
Locus At5g64070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25639380 of line 138D02 Chr5:25639380
   
Insertion allele amplicon 3144 - CL25
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer CL25: 5'-TGATACCGCTAATCTTCCTTGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25639784
   
wt amplicon (over InsPos) 24rd - CL25
wt amplicon start Chr5:25639060
wt amplicon end Chr5:25639784
wt amplicon size 725 bp
Locus specific primer CL25: 5'-TGATACCGCTAATCTTCCTTGTCT-3'
2nd locus specific primer 24rd: 5'-AACAGATGTGCAGAAACTTTGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11