Genotyping and primer details


Genotyping and primer details for 140F01 - At1g47271
Line ID 140F01
Locus At1g47271 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17329324 of line 140F01 Chr1:17329324
   
Insertion allele amplicon 8474 - 09oc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09oc: 5'-CAAAACCCAATCTCTGTTTCATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17328789
   
wt amplicon (over InsPos) 72rd - 09oc
wt amplicon start Chr1:17328789
wt amplicon end Chr1:17329598
wt amplicon size 810 bp
Locus specific primer 09oc: 5'-CAAAACCCAATCTCTGTTTCATTT-3'
2nd locus specific primer 72rd: 5'-GTTTTTAACACGACCAACGAGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11