Genotyping and primer details


Genotyping and primer details for 146D10 - At4g24860
Line ID 146D10
Locus At4g24860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12803103 of line 146D10 Chr4:12803103
   
Insertion allele amplicon 3144 - 19dh
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 19dh: 5'-AAAGATAACGCTAGGGGACATTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12802660
   
wt amplicon (over InsPos) 20dy - 19dh
wt amplicon start Chr4:12802660
wt amplicon end Chr4:12803562
wt amplicon size 903 bp
Locus specific primer 19dh: 5'-AAAGATAACGCTAGGGGACATTTT-3'
2nd locus specific primer 20dy: 5'-TATCTGTCTCGGTGTATTGAGTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11