Genotyping and primer details


Genotyping and primer details for 146D12 - At5g18120
Line ID 146D12
Locus At5g18120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5993159 of line 146D12 Chr5:5993159
   
Insertion allele amplicon 8474 - 05fm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05fm: 5'-CTTTCTTGGAAGTTCCTTGTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5993638
   
wt amplicon (over InsPos) 03gl - 05fm
wt amplicon start Chr5:5992596
wt amplicon end Chr5:5993638
wt amplicon size 1043 bp
Locus specific primer 05fm: 5'-CTTTCTTGGAAGTTCCTTGTTTTG-3'
2nd locus specific primer 03gl: 5'-CAATTGAACCATTCACAACAAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11