Genotyping and primer details


Genotyping and primer details for 146F03 - At2g01260
Line ID 146F03
Locus At2g01260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:136708 of line 146F03 Chr2:136708
   
Insertion allele amplicon 3144 - 10vd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 10vd: 5'-AACAGACTCAAACAAGTTCCCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:136304
   
wt amplicon (over InsPos) 32vw - 10vd
wt amplicon start Chr2:136304
wt amplicon end Chr2:137271
wt amplicon size 968 bp
Locus specific primer 10vd: 5'-AACAGACTCAAACAAGTTCCCAAT-3'
2nd locus specific primer 32vw: 5'-GGCTCAGTTTTTATCCAAGGTTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11