Genotyping and primer details


Genotyping and primer details for 146H11 - At1g26790
Line ID 146H11
Locus At1g26790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9275180 of line 146H11 Chr1:9275180
   
Insertion allele amplicon 8474 - IW05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer IW05: 5'-GAAACAGGTTCAGCGTTTATCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9274320
   
wt amplicon (over InsPos) 15dk - IW05
wt amplicon start Chr1:9274320
wt amplicon end Chr1:9275381
wt amplicon size 1062 bp
Locus specific primer IW05: 5'-GAAACAGGTTCAGCGTTTATCTTC-3'
2nd locus specific primer 15dk: 5'-TTAGTGGCACTGAAAGAGAGATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11