Genotyping and primer details


Genotyping and primer details for 148E10 - At4g20200
Line ID 148E10
Locus At4g20200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10910391 of line 148E10 Chr4:10910391
   
Insertion allele amplicon 3144 - 43xd
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 43xd: 5'-TAGTATACCTTTCCAGGCTGTTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10909718
   
Insertion allele amplicon 8474 - BK90
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BK90: 5'-TCGTTTGAAGGCCACTAATACTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10911017
   
wt amplicon (over InsPos) AP37 - BK90
wt amplicon start Chr4:10909801
wt amplicon end Chr4:10911017
wt amplicon size 1217 bp
Locus specific primer BK90: 5'-TCGTTTGAAGGCCACTAATACTCT-3'
2nd locus specific primer AP37: 5'-AAACCGGTGAAGTACTGAATCATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11