Genotyping and primer details


Genotyping and primer details for 149D06 - At1g19330
Line ID 149D06
Locus At1g19330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6682384 of line 149D06 Chr1:6682384
   
Insertion allele amplicon 8474 - 04um
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04um: 5'-TGTTACTTCCATAGGAATTCTGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6681902
   
wt amplicon (over InsPos) 29vg - 04um
wt amplicon start Chr1:6681902
wt amplicon end Chr1:6682645
wt amplicon size 744 bp
Locus specific primer 04um: 5'-TGTTACTTCCATAGGAATTCTGGG-3'
2nd locus specific primer 29vg: 5'-TCACCATTATTGCTGGATTTTACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11