Genotyping and primer details


Genotyping and primer details for 153C02 - At4g00820
Line ID 153C02
Locus At4g00820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:350696 of line 153C02 Chr4:350696
   
Insertion allele amplicon 3144 - 11uw
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 11uw: 5'-TAAGTTTCAGAACACCATTGTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:350198
   
wt amplicon (over InsPos) 43vl - 11uw
wt amplicon start Chr4:350198
wt amplicon end Chr4:351187
wt amplicon size 990 bp
Locus specific primer 11uw: 5'-TAAGTTTCAGAACACCATTGTTGC-3'
2nd locus specific primer 43vl: 5'-GCCACCTATACTCTTGAAACTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11