Genotyping and primer details


Genotyping and primer details for 155D01 - At5g16290
Line ID 155D01
Locus At5g16290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5335088 of line 155D01 Chr5:5335088
   
Insertion allele amplicon 8474 - 10et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10et: 5'-GATTTGCAGAGTAAGGAGGCATAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5334553
   
wt amplicon (over InsPos) 13fo - 10et
wt amplicon start Chr5:5334553
wt amplicon end Chr5:5335638
wt amplicon size 1086 bp
Locus specific primer 10et: 5'-GATTTGCAGAGTAAGGAGGCATAC-3'
2nd locus specific primer 13fo: 5'-ACAGTTAAGAAGGATTGGAGCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11