Genotyping and primer details


Genotyping and primer details for 156E10 - At1g18900
Line ID 156E10
Locus At1g18900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6532322 of line 156E10 Chr1:6532322
   
Insertion allele amplicon 8474 - 01cv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01cv: 5'-AAAACTTGATATGGGTTTCTGTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6531754
   
Insertion allele amplicon 8474 - 13dh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13dh: 5'-TGTGTAATGACTGTGGAAGAAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6532800
   
Insertion allele amplicon 8474 - 28dj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28dj: 5'-CACATATGTTTCTCCTCAAGATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6531807
   
wt amplicon (over InsPos) 13dh - 01cv
wt amplicon start Chr1:6531754
wt amplicon end Chr1:6532800
wt amplicon size 1046 bp
Locus specific primer 01cv: 5'-AAAACTTGATATGGGTTTCTGTGG-3'
2nd locus specific primer 13dh: 5'-TGTGTAATGACTGTGGAAGAAAGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11