Genotyping and primer details


Genotyping and primer details for 165E10 - At5g08600
Line ID 165E10
Locus At5g08600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2788515 of line 165E10 Chr5:2788515
   
Insertion allele amplicon 8474 - 03md
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03md: 5'-CAAATTCATGCTACTTTGTCCAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2787912
   
wt amplicon (over InsPos) 03nd - 03md
wt amplicon start Chr5:2787912
wt amplicon end Chr5:2788818
wt amplicon size 907 bp
Locus specific primer 03md: 5'-CAAATTCATGCTACTTTGTCCAGA-3'
2nd locus specific primer 03nd: 5'-TTCCATTGTGATTTGAAGAAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11