Genotyping and primer details


Genotyping and primer details for 165G06 - At3g16040
Line ID 165G06
Locus At3g16040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5443684 of line 165G06 Chr3:5443684
   
Insertion allele amplicon 8474 - 05qw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05qw: 5'-GAAAAGTTGCAGAATTTTTGAAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5443970
   
wt amplicon (over InsPos) 04rt - 05qw
wt amplicon start Chr3:5443123
wt amplicon end Chr3:5443970
wt amplicon size 848 bp
Locus specific primer 05qw: 5'-GAAAAGTTGCAGAATTTTTGAAGG-3'
2nd locus specific primer 04rt: 5'-GTGTTTTTGTTTTGGGATTAAGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11