Genotyping and primer details


Genotyping and primer details for 168E01 - At3g16340
Line ID 168E01
Locus At3g16340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5543116 of line 168E01 Chr3:5543116
   
Insertion allele amplicon 8474 - 26ci
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 26ci: 5'-TAGGAATGTAAGAGCGAGGGTAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5543579
   
wt amplicon (over InsPos) 05cm - 26ci
wt amplicon start Chr3:5542819
wt amplicon end Chr3:5543579
wt amplicon size 761 bp
Locus specific primer 26ci: 5'-TAGGAATGTAAGAGCGAGGGTAAC-3'
2nd locus specific primer 05cm: 5'-TGTTGTTTGGGTCACCATTACTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11