Genotyping and primer details


Genotyping and primer details for 169A01 - At5g59390
Line ID 169A01
Locus At5g59390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23957244 of line 169A01 Chr5:23957244
   
Insertion allele amplicon 8474 - 08uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08uj: 5'-CAATTCTCAAAAGATGGGGTTTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23956816
   
wt amplicon (over InsPos) 18vl - 08uj
wt amplicon start Chr5:23956816
wt amplicon end Chr5:23957281
wt amplicon size 466 bp
Locus specific primer 08uj: 5'-CAATTCTCAAAAGATGGGGTTTTA-3'
2nd locus specific primer 18vl: 5'-GAAGAAACAAAATCCAATTCGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11