Genotyping and primer details


Genotyping and primer details for 169H04 - At5g43930
Line ID 169H04
Locus At5g43930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17679518 of line 169H04 Chr5:17679518
   
Insertion allele amplicon 8474 - 21kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21kn: 5'-CAAATAGATCAGATTTCCCTGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17679000
   
wt amplicon (over InsPos) 11lb - 21kn
wt amplicon start Chr5:17679000
wt amplicon end Chr5:17680024
wt amplicon size 1025 bp
Locus specific primer 21kn: 5'-CAAATAGATCAGATTTCCCTGAGC-3'
2nd locus specific primer 11lb: 5'-AATCTGATTATTCTCAAAAGGCGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11