Genotyping and primer details


Genotyping and primer details for 171C11 - At5g16030
Line ID 171C11
Locus At5g16030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5239152 of line 171C11 Chr5:5239152
   
Insertion allele amplicon 8474 - 04mh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 04mh: 5'-ACAATAACAGGGAATGGTTAGCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5238704
   
wt amplicon (over InsPos) 02nd - 04mh
wt amplicon start Chr5:5238704
wt amplicon end Chr5:5239698
wt amplicon size 995 bp
Locus specific primer 04mh: 5'-ACAATAACAGGGAATGGTTAGCAC-3'
2nd locus specific primer 02nd: 5'-TGTCTCTTTCCATGAAAACATTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11