Genotyping and primer details


Genotyping and primer details for 175D03 - At2g34840
Line ID 175D03
Locus At2g34840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14703375 of line 175D03 Chr2:14703375
   
Insertion allele amplicon 8474 - 14df
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14df: 5'-GCTACTCCACTACAAGCAGTCAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14702899
   
Insertion allele amplicon 8474 - 75cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 75cs: 5'-CCTCGCTATGTATCTATCGACTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14702925
   
wt amplicon (over InsPos) 24de - 75cs
wt amplicon start Chr2:14702925
wt amplicon end Chr2:14703778
wt amplicon size 854 bp
Locus specific primer 75cs: 5'-CCTCGCTATGTATCTATCGACTCC-3'
2nd locus specific primer 24de: 5'-AAGAGACAAGTGCAGTGTGAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11