Genotyping and primer details


Genotyping and primer details for 175D10 - At3g20760
Line ID 175D10
Locus At3g20760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7257725 of line 175D10 Chr3:7257725
   
Insertion allele amplicon 8474 - 30ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30ra: 5'-AACCTTGTTTCCTCGACAGTTCTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7257270
   
wt amplicon (over InsPos) 05rz - 30ra
wt amplicon start Chr3:7257270
wt amplicon end Chr3:7258214
wt amplicon size 945 bp
Locus specific primer 30ra: 5'-AACCTTGTTTCCTCGACAGTTCTA-3'
2nd locus specific primer 05rz: 5'-AGCTGAGTTTGTTAATGCCTTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11