Genotyping and primer details


Genotyping and primer details for 179A06 - At1g71360
Line ID 179A06
Locus At1g71360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26892954 of line 179A06 Chr1:26892954
   
Insertion allele amplicon 8474 - 05wm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05wm: 5'-TTCTCTTTCTCATTCTCAGCCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26892428
   
wt amplicon (over InsPos) 28xd - 05wm
wt amplicon start Chr1:26892428
wt amplicon end Chr1:26893550
wt amplicon size 1123 bp
Locus specific primer 05wm: 5'-TTCTCTTTCTCATTCTCAGCCTTC-3'
2nd locus specific primer 28xd: 5'-ATACAGAGACCACTGGTTCAAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11