Genotyping and primer details


Genotyping and primer details for 179C08 - At3g15710
Line ID 179C08
Locus At3g15710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5324362 of line 179C08 Chr3:5324362
   
Insertion allele amplicon 8474 - 10ic
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10ic: 5'-CAATTGATTTTACTCTCATTTGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5324692
   
wt amplicon (over InsPos) 02il - 10ic
wt amplicon start Chr3:5323770
wt amplicon end Chr3:5324692
wt amplicon size 923 bp
Locus specific primer 10ic: 5'-CAATTGATTTTACTCTCATTTGCG-3'
2nd locus specific primer 02il: 5'-TCTGAAAAGAGATCAAAAGGGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11