Genotyping and primer details


Genotyping and primer details for 179C09 - At3g09360
Line ID 179C09
Locus At3g09360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2876609 of line 179C09 Chr3:2876609
   
Insertion allele amplicon 8474 - 41fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 41fr: 5'-TTAAAAGTTCGGCACAAGAAAGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2876939
   
wt amplicon (over InsPos) 20go - 41fr
wt amplicon start Chr3:2876190
wt amplicon end Chr3:2876939
wt amplicon size 750 bp
Locus specific primer 41fr: 5'-TTAAAAGTTCGGCACAAGAAAGAT-3'
2nd locus specific primer 20go: 5'-GCAGAATTACATGCTATCTGGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11