Genotyping and primer details


Genotyping and primer details for 191C09 - At5g01950
Line ID 191C09
Locus At5g01950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:368357 of line 191C09 Chr5:368357
   
Insertion allele amplicon 8474 - NH55
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer NH55: 5'-AAAAGGCTTTACTTTTGGGAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:369201
   
wt amplicon (over InsPos) MN51 - NH55
wt amplicon start Chr5:368188
wt amplicon end Chr5:369201
wt amplicon size 1014 bp
Locus specific primer NH55: 5'-AAAAGGCTTTACTTTTGGGAGAAG-3'
2nd locus specific primer MN51: 5'-CCTGAAATTGAGGACAATAACCAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11