Genotyping and primer details


Genotyping and primer details for 191C11 - At3g61310
Line ID 191C11
Locus At3g61310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22691532 of line 191C11 Chr3:22691532
   
Insertion allele amplicon 8474 - 07nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07nt: 5'-GTTGGCATTGTCTCCTTCTATCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22692092
   
wt amplicon (over InsPos) 27oj - 07nt
wt amplicon start Chr3:22691190
wt amplicon end Chr3:22692092
wt amplicon size 903 bp
Locus specific primer 07nt: 5'-GTTGGCATTGTCTCCTTCTATCTC-3'
2nd locus specific primer 27oj: 5'-CTAGCAAGTGAGACCGCTAGACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11