Genotyping and primer details


Genotyping and primer details for 197E08 - At1g61140
Line ID 197E08
Locus At1g61140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22538537 of line 197E08 Chr1:22538537
   
Insertion allele amplicon 8474 - HP15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer HP15: 5'-AACAGCTCTACCTAGCCAAGACAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22538965
   
wt amplicon (over InsPos) 05si - HP15
wt amplicon start Chr1:22538059
wt amplicon end Chr1:22538965
wt amplicon size 907 bp
Locus specific primer HP15: 5'-AACAGCTCTACCTAGCCAAGACAG-3'
2nd locus specific primer 05si: 5'-GCTGGGAAAGATATCAACAGATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11