Genotyping and primer details


Genotyping and primer details for 200G05 - At1g66740
Line ID 200G05
Locus At1g66740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24893105 of line 200G05 Chr1:24893105
   
Insertion allele amplicon 8474 - MK79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MK79: 5'-GCATAATCCTGCTCCTTTTGTTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24892621
   
wt amplicon (over InsPos) 03mx - MK79
wt amplicon start Chr1:24892621
wt amplicon end Chr1:24893726
wt amplicon size 1106 bp
Locus specific primer MK79: 5'-GCATAATCCTGCTCCTTTTGTTAG-3'
2nd locus specific primer 03mx: 5'-TTTGCCCAAAACATAAACATACAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11