Genotyping and primer details


Genotyping and primer details for 204C07 - At5g61790
Line ID 204C07
Locus At5g61790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24829217 of line 204C07 Chr5:24829217
   
Insertion allele amplicon 8474 - 11aq
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11aq: 5'-GTCTTCACCAGAGAGTAAATTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24828610
   
Insertion allele amplicon 3144 - 11bg
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 11bg: 5'-TTTGCTTCTCTTAGCTTTCGTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24829613
   
wt amplicon (over InsPos) 11bg - 11aq
wt amplicon start Chr5:24828610
wt amplicon end Chr5:24829613
wt amplicon size 1004 bp
Locus specific primer 11aq: 5'-GTCTTCACCAGAGAGTAAATTGGC-3'
2nd locus specific primer 11bg: 5'-TTTGCTTCTCTTAGCTTTCGTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11