Genotyping and primer details


Genotyping and primer details for 205G10 - At2g45930
Line ID 205G10
Locus At2g45930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18902566 of line 205G10 Chr2:18902566
   
Insertion allele amplicon 8474 - 14sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 14sj: 5'-GATCTCAAGTCCTAAACAATTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18902149
   
wt amplicon (over InsPos) 06ti - 14sj
wt amplicon start Chr2:18902149
wt amplicon end Chr2:18903161
wt amplicon size 1013 bp
Locus specific primer 14sj: 5'-GATCTCAAGTCCTAAACAATTGGC-3'
2nd locus specific primer 06ti: 5'-ACTTAACCAACTCTTTGGGAAGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11