Genotyping and primer details


Genotyping and primer details for 208D03 - At3g16180
Line ID 208D03
Locus At3g16180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5484302 of line 208D03 Chr3:5484302
   
Insertion allele amplicon 8474 - 13de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 13de: 5'-AGGACAAACTGTCTTGTTCATGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5484663
   
wt amplicon (over InsPos) 26dy - 13de
wt amplicon start Chr3:5483997
wt amplicon end Chr3:5484663
wt amplicon size 667 bp
Locus specific primer 13de: 5'-AGGACAAACTGTCTTGTTCATGTG-3'
2nd locus specific primer 26dy: 5'-GATTTAGCAAAACCAACGGTAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11