Genotyping and primer details


Genotyping and primer details for 213D06 - At4g35460
Line ID 213D06
Locus At4g35460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:16843410 of line 213D06 Chr4:16843410
   
Insertion allele amplicon 8474 - AU10
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AU10: 5'-CAATACCCGTACTCAATTCCAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:16843905
   
wt amplicon (over InsPos) 32si - AU10
wt amplicon start Chr4:16842986
wt amplicon end Chr4:16843905
wt amplicon size 920 bp
Locus specific primer AU10: 5'-CAATACCCGTACTCAATTCCAACT-3'
2nd locus specific primer 32si: 5'-AACTCGTCTGTTGTGGAAGCTTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11