Genotyping and primer details


Genotyping and primer details for 214F01 - At2g31810
Line ID 214F01
Locus At2g31810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13526637 of line 214F01 Chr2:13526637
   
Insertion allele amplicon 8474 - 51et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 51et: 5'-GCCATCAAGACATTCAAACTGTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13527165
   
wt amplicon (over InsPos) 19fg - 51et
wt amplicon start Chr2:13526156
wt amplicon end Chr2:13527165
wt amplicon size 1010 bp
Locus specific primer 51et: 5'-GCCATCAAGACATTCAAACTGTTA-3'
2nd locus specific primer 19fg: 5'-GTGTGCTTCTCTGCTCCTTAGATT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11