Genotyping and primer details


Genotyping and primer details for 215D03 - At1g77220
Line ID 215D03
Locus At1g77220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29013663 of line 215D03 Chr1:29013663
   
Insertion allele amplicon 8474 - 02im
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02im: 5'-CCAAGACTCCAATCTTTTACGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29014240
   
wt amplicon (over InsPos) 03pc - 02im
wt amplicon start Chr1:29013396
wt amplicon end Chr1:29014240
wt amplicon size 845 bp
Locus specific primer 02im: 5'-CCAAGACTCCAATCTTTTACGAAG-3'
2nd locus specific primer 03pc: 5'-CCTCCCCATGTATCTTATCTTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11