Genotyping and primer details


Genotyping and primer details for 215E10 - At3g53500
Line ID 215E10
Locus At3g53500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19835833 of line 215E10 Chr3:19835833
   
Insertion allele amplicon 8474 - KS24
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KS24: 5'-TAGTACCTGGCCTTCTTTGGACTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19835364
   
wt amplicon (over InsPos) 08gy - KS24
wt amplicon start Chr3:19835364
wt amplicon end Chr3:19836189
wt amplicon size 826 bp
Locus specific primer KS24: 5'-TAGTACCTGGCCTTCTTTGGACTA-3'
2nd locus specific primer 08gy: 5'-AATTGTGGAGCTAGGTTTAGTGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11