Genotyping and primer details


Genotyping and primer details for 218D01 - At1g07280
Line ID 218D01
Locus At1g07280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2239797 of line 218D01 Chr1:2239797
   
Insertion allele amplicon 3144 - AL07
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer AL07: 5'-CTTCTCGCTAATTTCACGGTTTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2239330
   
Insertion allele amplicon 8474 - KA30
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KA30: 5'-AAAGCCTATCCGATTGGTTCTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2240169
   
wt amplicon (over InsPos) AL07 - KA30
wt amplicon start Chr1:2239330
wt amplicon end Chr1:2240169
wt amplicon size 840 bp
Locus specific primer KA30: 5'-AAAGCCTATCCGATTGGTTCTTAT-3'
2nd locus specific primer AL07: 5'-CTTCTCGCTAATTTCACGGTTTAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11