Genotyping and primer details


Genotyping and primer details for 223C06 - At1g62960
Line ID 223C06
Locus At1g62960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23318407 of line 223C06 Chr1:23318407
   
Insertion allele amplicon 8474 - 17db
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17db: 5'-TTTGACAGAAGATTGAGAGGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23317909
   
wt amplicon (over InsPos) 12mx - 17db
wt amplicon start Chr1:23317909
wt amplicon end Chr1:23318842
wt amplicon size 934 bp
Locus specific primer 17db: 5'-TTTGACAGAAGATTGAGAGGGTTT-3'
2nd locus specific primer 12mx: 5'-TTGTTAGCATGGCTGAAATAGTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11