Genotyping and primer details


Genotyping and primer details for 224G05 - At4g14410
Line ID 224G05
Locus At4g14410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8301339 of line 224G05 Chr4:8301339
   
Insertion allele amplicon 8474 - h104
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer h104: 5'-GCTGTTAAGGAAGAACAGGAACAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8300715
   
wt amplicon (over InsPos) 02id - h104
wt amplicon start Chr4:8300715
wt amplicon end Chr4:8301877
wt amplicon size 1163 bp
Locus specific primer h104: 5'-GCTGTTAAGGAAGAACAGGAACAT-3'
2nd locus specific primer 02id: 5'-GTCCTAATTGGCTAATTTGTTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11