Genotyping and primer details


Genotyping and primer details for 225B03 - At5g66560
Line ID 225B03
Locus At5g66560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26565063 of line 225B03 Chr5:26565063
   
Insertion allele amplicon 8474 - 11uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11uj: 5'-TTGTTGAGGAGATGGAGCTTTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26565597
   
wt amplicon (over InsPos) 22vg - 11uj
wt amplicon start Chr5:26564555
wt amplicon end Chr5:26565597
wt amplicon size 1043 bp
Locus specific primer 11uj: 5'-TTGTTGAGGAGATGGAGCTTTTAT-3'
2nd locus specific primer 22vg: 5'-ATGACATGACATTCCATCTCCATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11