Genotyping and primer details


Genotyping and primer details for 228F07 - At5g40100
Line ID 228F07
Locus At5g40100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16044923 of line 228F07 Chr5:16044923
   
Insertion allele amplicon 8474 - 18lm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18lm: 5'-TCTCTGCTAAAACTCTCATCCACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16045471
   
wt amplicon (over InsPos) 01mr - 18lm
wt amplicon start Chr5:16044445
wt amplicon end Chr5:16045471
wt amplicon size 1027 bp
Locus specific primer 18lm: 5'-TCTCTGCTAAAACTCTCATCCACA-3'
2nd locus specific primer 01mr: 5'-ATGTATTTTTCCTCGCACAAGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11