Genotyping and primer details


Genotyping and primer details for 230B08 - At4g30100
Line ID 230B08
Locus At4g30100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14717815 of line 230B08 Chr4:14717815
   
Insertion allele amplicon 8474 - 38ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 38ra: 5'-GTTCCAGCAGCTTTGCTAATAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14718261
   
wt amplicon (over InsPos) 44sq - 38ra
wt amplicon start Chr4:14717515
wt amplicon end Chr4:14718261
wt amplicon size 747 bp
Locus specific primer 38ra: 5'-GTTCCAGCAGCTTTGCTAATAACT-3'
2nd locus specific primer 44sq: 5'-AAAACTGTGTGCTAAACCAAGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11