Genotyping and primer details


Genotyping and primer details for 230E11 - At1g51520
Line ID 230E11
Locus At1g51520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19107333 of line 230E11 Chr1:19107333
   
Insertion allele amplicon 8474 - 07ea
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07ea: 5'-TTTTCTCTTCGTCTTCTTCACCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19106735
   
wt amplicon (over InsPos) 02fd - 07ea
wt amplicon start Chr1:19106735
wt amplicon end Chr1:19107697
wt amplicon size 963 bp
Locus specific primer 07ea: 5'-TTTTCTCTTCGTCTTCTTCACCAT-3'
2nd locus specific primer 02fd: 5'-AGAAACAATCACCATGAGGAAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11