Genotyping and primer details


Genotyping and primer details for 232G05 - At1g56385
Line ID 232G05
Locus At1g56385 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21106788 of line 232G05 Chr1:21106788
   
Insertion allele amplicon 8474 - 45oh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45oh: 5'-GCTCTAAATTTGAAGTGGGTGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21106159
   
wt amplicon (over InsPos) 05rd - 45oh
wt amplicon start Chr1:21106159
wt amplicon end Chr1:21107160
wt amplicon size 1002 bp
Locus specific primer 45oh: 5'-GCTCTAAATTTGAAGTGGGTGGTA-3'
2nd locus specific primer 05rd: 5'-CGTTTTTAGCGAAGATTTTAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11