Genotyping and primer details


Genotyping and primer details for 233H09 - At1g53040
Line ID 233H09
Locus At1g53040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19764938 of line 233H09 Chr1:19764938
   
Insertion allele amplicon 8474 - 38vw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 38vw: 5'-TACAGACGATAACAAGAGAGTGGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19765669
   
Insertion allele amplicon 8474 - 45vr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45vr: 5'-TTGGTTCTTTTGACAGAAACTCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19764395
   
wt amplicon (over InsPos) 38vw - 45vr
wt amplicon start Chr1:19764395
wt amplicon end Chr1:19765669
wt amplicon size 1275 bp
Locus specific primer 45vr: 5'-TTGGTTCTTTTGACAGAAACTCAC-3'
2nd locus specific primer 38vw: 5'-TACAGACGATAACAAGAGAGTGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11